Yes, you can pay $50,000 to clone a pet. But others are using the technology to rescue endangered species. This week, we heard that Tom Brady had his dog cloned. The former quarterback revealed that his Junie is actually a clone of Lua, a pit bull mix that died in 2023. Brady's announcement follows those of celebrities like Paris Hilton and Barbra Streisand, who also famously cloned their pet dogs. But some believe there are better ways to make use of cloning technologies.

While Whole Slide Imaging (WSI) scanners remain the gold standard for digitizing pathology samples, their high cost limits accessibility in many healthcare settings. Other low-cost solutions also face critical limitations: automated microscopes struggle with consistent focus across varying tissue morphology, traditional auto-focus methods require time-consuming focal stacks, and existing deep-learning approaches either need multiple input images or lack generalization capability across tissue types and staining protocols. We introduce a novel automated microscopic system powered by DeepAf, a novel auto-focus framework that uniquely combines spatial and spectral features through a hybrid architecture for single-shot focus prediction. The proposed network automatically regresses the distance to the optimal focal point using the extracted spatiospectral features and adjusts the control parameters for optimal image outcomes. Our system transforms conventional microscopes into efficient slide scanners, reducing focusing time by 80% compared to stack-based methods while achieving focus accuracy of 0.18 μm on the same-lab samples, matching the performance of dual-image methods (0.19 μm) with half the input requirements. DeepAf demonstrates robust cross-lab generalization with only 0.72% false focus predictions and 90% of predictions within the depth of field. Through an extensive clinical study of 536 brain tissue samples, our system achieves 0.90 AUC in cancer classification at 4x magnification, a significant achievement at lower magnification than typical 20x WSI scans. This results in a comprehensive hardware-software design enabling accessible, real-time digital pathology in resource-constrained settings while maintaining diagnostic accuracy.

Glioblastoma, a highly aggressive brain tumor with diverse molecular and pathological features, poses a diagnostic challenge due to its heterogeneity. Accurate diagnosis and assessment of this heterogeneity are essential for choosing the right treatment and improving patient outcomes. Traditional methods rely on identifying specific features in tissue samples, but deep learning offers a promising approach for improved glioblastoma diagnosis. In this paper, we present our approach to the BraTS-Path Challenge 2024. We leverage a pre-trained model and fine-tune it on the BraTS-Path training dataset. Our model demonstrates poor performance on the challenging BraTS-Path validation set, as rigorously assessed by the Synapse online platform. The model achieves an accuracy of 0.392229, a recall of 0.392229, and a F1-score of 0.392229, indicating a consistent ability to correctly identify instances under the target condition. Notably, our model exhibits perfect specificity of 0.898704, showing an exceptional capacity to correctly classify negative cases. Moreover, a Matthews Correlation Coefficient (MCC) of 0.255267 is calculated, to signify a limited positive correlation between predicted and actual values and highlight our model's overall predictive power. Our solution also achieves the second place during the testing phase.

Transluminal minimally invasive surgery uses natural orifices and small incisions to access internal anatomical structures, promoting quicker recovery and reduced morbidity. However, navigating instruments--catheters and endoscopes--through anatomical pathways creates frictional interactions with luminal walls, risking complications such as perforation, poor haptic feedback, and instrument buckling. In this paper, we present a new approach to actively lubricate transluminal instruments and dynamically reduce friction with surrounding tissues. This approach employs ultrasonic vibrations, at the instrument surface, to generate a pressurized fluid layer at the contact interface, lubricating the interface and thereby reducing friction. We implemented this approach in a prototype catheter, which we validated under dry and liquid-lubricated conditions, across rigid and soft interfaces, and along varied anatomical curvatures. In a cardiac catheter use case, active lubrication reduced friction by up to 42% on ex-vivo porcine aorta tissue and 82% on rigid substrates, denoting its potential performance on healthy and calcified tissue, respectively. Thermal imaging confirmed that temperature at the tissue-catheter interface remained within safe limits. Additionally, the system effectively prevented buckling during catheter insertion experiment, further showcasing its potential. By minimizing injury risk and enhancing procedural stability, active lubrication can drastically enhance the safety and efficacy of transluminal interventions.

In theory, artificial intelligence should be great at helping out. "Our job is pattern recognition," says Andrew Norgan, a pathologist and medical director of the Mayo Clinic's digital pathology platform. "We look at the slide and we gather pieces of information that have been proven to be important." Visual analysis is something that AI has gotten quite good at since the first image recognition models began taking off nearly 15 years ago. Even though no model will be perfect, you can imagine a powerful algorithm someday catching something that a human pathologist missed, or at least speeding up the process of getting a diagnosis.

This work demonstrates the benefits of using tool-tissue interaction forces in the design of autonomous systems in robot-assisted surgery (RAS). Autonomous systems in surgery must manipulate tissues of different stiffness levels and hence should apply different levels of forces accordingly. We hypothesize that this ability is enabled by using force measurements as input to policies learned from human demonstrations. To test this hypothesis, we use Action-Chunking Transformers (ACT) to train two policies through imitation learning for automated tissue retraction with the da Vinci Research Kit (dVRK). To quantify the effects of using tool-tissue interaction force data, we trained a "no force policy" that uses the vision and robot kinematic data, and compared it to a "force policy" that uses force, vision and robot kinematic data. When tested on a previously seen tissue sample, the force policy is 3 times more successful in autonomously performing the task compared with the no force policy. In addition, the force policy is more gentle with the tissue compared with the no force policy, exerting on average 62% less force on the tissue. When tested on a previously unseen tissue sample, the force policy is 3.5 times more successful in autonomously performing the task, exerting an order of magnitude less forces on the tissue, compared with the no force policy. These results open the door to design force-aware autonomous systems that can meet the surgical guidelines for tissue handling, especially using the newly released RAS systems with force feedback capabilities such as the da Vinci 5.

Purpose: This work addresses the detection of Helicobacter pylori (H. pylori) in histological images with immunohistochemical staining. This analysis is a time demanding task, currently done by an expert pathologist that visually inspects the samples. Given the effort required to localise the pathogen in images, a limited number of annotations might be available in an initial setting. Our goal is to design an approach that, using a limited set of annotations, is capable of obtaining results good enough to be used as a support tool. Methods: We propose to use autoencoders to learn the latent patterns of healthy patches and formulate a specific measure of the reconstruction error of the image in HSV space. ROC analysis is used to set the optimal threshold of this measure and the percentage of positive patches in a sample that determines the presence of H. pylori. Results: Our method has been tested on an own database of 245 Whole Slide Images (WSI) having 117 cases without H. pylori and different density of the bacteria in the remaining ones. The database has 1211 annotated patches, with only 163 positive patches. This dataset of positive annotations was used to train a baseline thresholding and an SVM using the features of a pre-trained RedNet18 and ViT models. A 10-fold cross-validation shows that our method has better performance with 91% accuracy, 86% sensitivity, 96% specificity and 0.97 AUC in the diagnosis of H. pylori. Conclusion: Unlike classification approaches, our shallow autoencoder with threshold adaptation for the detection of anomalous staining is able to achieve competitive results with a limited set of annotated data. This initial approach is good enough to be used as a guide for fast annotation of infected patches.

Virtual staining of tissue offers a powerful tool for transforming label-free microscopy images of unstained tissue into equivalents of histochemically stained samples. This study presents a diffusion model-based super-resolution virtual staining approach utilizing a Brownian bridge process to enhance both the spatial resolution and fidelity of label-free virtual tissue staining, addressing the limitations of traditional deep learning-based methods. Our approach integrates novel sampling techniques into a diffusion model-based image inference process to significantly reduce the variance in the generated virtually stained images, resulting in more stable and accurate outputs. Blindly applied to lower-resolution auto-fluorescence images of label-free human lung tissue samples, the diffusion-based super-resolution virtual staining model consistently outperformed conventional approaches in resolution, structural similarity and perceptual accuracy, successfully achieving a super-resolution factor of 4-5x, increasing the output space-bandwidth product by 16-25-fold compared to the input label-free microscopy images. Diffusion-based super-resolved virtual tissue staining not only improves resolution and image quality but also enhances the reliability of virtual staining without traditional chemical staining, offering significant potential for clinical diagnostics.

Histopathological staining of human tissue is essential in the diagnosis of various diseases. The recent advances in virtual tissue staining technologies using AI alleviate some of the costly and tedious steps involved in the traditional histochemical staining process, permitting multiplexed rapid staining of label-free tissue without using staining reagents, while also preserving tissue. However, potential hallucinations and artifacts in these virtually stained tissue images pose concerns, especially for the clinical utility of these approaches. Quality assessment of histology images is generally performed by human experts, which can be subjective and depends on the training level of the expert. Here, we present an autonomous quality and hallucination assessment method (termed AQuA), mainly designed for virtual tissue staining, while also being applicable to histochemical staining. AQuA achieves 99.8% accuracy when detecting acceptable and unacceptable virtually stained tissue images without access to ground truth, also presenting an agreement of 98.5% with the manual assessments made by board-certified pathologists. Besides, AQuA achieves super-human performance in identifying realistic-looking, virtually stained hallucinatory images that would normally mislead human diagnosticians by deceiving them into diagnosing patients that never existed. We further demonstrate the wide adaptability of AQuA across various virtually and histochemically stained tissue images and showcase its strong external generalization to detect unseen hallucination patterns of virtual staining network models as well as artifacts observed in the traditional histochemical staining workflow. This framework creates new opportunities to enhance the reliability of virtual staining and will provide quality assurance for various image generation and transformation tasks in digital pathology and computational imaging.

A robotic dodecahedron can capture fragile deep-sea animals to collect tissues samples and construct three-dimensional scans of the creatures, potentially speeding up the cataloguing of the up to 66 per cent of ocean species that are yet to be described by science. Brennan Phillips at the University of Rhode Island and his colleagues developed the RAD2 sampler, designed to mount on any submersible, to collect fresh tissue samples from living animals in situ. They hope this will reveal more about the creatures than existing techniques, which typically put them under stress as they are hauled from the depths. RAD2 is a dodecahedron with an internal volume large enough to hold a basketball. It is designed to fold and unfold on command to temporarily capture creatures for closer examination, taking a small tissue sample that is preserved directly on the submersible for later genetic analysis.